Speaker
Description
Light-sheet fluorescence microscopy provides rapid volumetric imaging of biological specimens with reduced photodamage by confining excitation to a thin optical plane. Combined with two-photon excitation fluorescence (2PEF), it enables deeper imaging with reduced background, making it well-suited to scattering samples.
Past nonlinear light-sheet implementations are limited by non-uniform illumination, shadowing, reliance on proprietary reconstruction software, and limited validation on biological specimens.
Here, we present a 2PEF light-sheet microscopy framework that addresses these limitations. An open-source reconstruction pipeline was developed, incorporating calculated point spread functions and multi-view deconvolution for flexible and accessible image processing. Rotational multi-view acquisition mitigates illumination non-uniformity and shadowing, improving volumetric coverage. Together, these features enable more robust volumetric reconstruction in scattering specimens.
Initial measurements on fluorescent beads and biological samples, including cardiomyoblasts and glioblastoma cells, will be presented to quantify resolution, contrast, and isotropy, thereby establishing a basis for assessing performance gains achieved through multi-view reconstruction.
| Apply for student award at which level: | MSc |
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| Consent on use of personal information: Abstract Submission | Yes, I ACCEPT |